Saturday, September 19, 2009

E-M34: Designation as "African" presents a Dilemma?

Earlier on this year, the present author of this site had an exchange with a chat room regular and the author of a paper titled "Y Haplogroups, Archaeological Cultures and Language Families: a Review of the Possibility of Multidisciplinary Comparisons Using the Case of E-M35", going by the name of Andrew Lancaster, on issues pertaining to the origins and demic diffusion possibilities of E1b1b subclade 'E-M34'. There are some observers out there who basically prefer to treat E-M34 as an isolated [standalone] lineage on its own, away from all its other E-M35 siblings, and mute the fact that this lineage essentially signifies recent common African ancestry. In what appears to be ironic, sections of these same observes are inclined to use E-M34 as primarily a marker of "external gene flow" into Africa. To put the underlying reasoning by such observers to test, the present author of this site decided to query the aforementioned Lancaster on his views.

In the mentioned paper, Lancaster sees the E-M34 clade more as ultimately a marker of demic diffusion "into Africa" as opposed to "out of Africa"; he invokes geneticists Cruciani et al. (2004) as his primary reference-sources, to justify his viewpoints. Here, he recites the claim that from a distribution standpoint, E-M34 clusters appear to be absent in nations immediately neighboring Ethiopia [like Sudan], and the supposed greater intra-clade diversity found in the Arabian peninsula than in Ethiopia. When repeatedly told that the geographical location of the contemporary nation of Ethiopia need not be the original African territory of the African forerunners of E-M34 carrying Ethiopians, or that for E-M34 to be deemed "African", it need not be predicated on it having to have originated in what is now Ethiopia, and that instead these forerunners could have originated in a more northern latitude in the geographical sphere of the eastern Sahara, Lancaster's reaction is to present what is now part of the contemporary nation of Egypt as a "concession" on his part. According to this "concession", he is willing to give into the possibility that E-M34 originated in what is now part of Egypt and thereafter spilled over to the Levant to its east, and coastal north African areas to its west. He points to the relatively visible, not necessarily predominant, distribution of E-M34 markers in these areas, and in some cases, sporadic incidences of paraphyletic examples of E-M34. Lancaster seems to seize on the condition of "Semitic" speaking in Ethiopia as something that further justifies his viewpoint.

Lancaster's so-called "concession" revolving around an Egyptian origin is all fine and dandy, but even here, he refuses to see an Egyptian origin as essentially an "African" origin as well. He refuses to get in touch with the reality that Egypt is part of Africa, and therefore, that the two are not mutually exclusive of one another. Logic intimates that what is Egyptian is by default also African, but for some reason, when it comes to things African, such no-brainers suddenly belie logic. He insists that it would be better [on his account] to perceive Egypt in terms of the over-time-changing and subjective Eurocentric-created geopolitical constructs like the "Middle East" rather than the less subjective designator for the continent to which Egypt belongs. In other words, his "concession" has a precondition attached to it; that is, as long as Egypt is viewed as "Middle East", which is tacitly supposed to be mutually exclusive of "Africa". It is against this backdrop, he chooses to interpret an African origin as needlessly implicating "all of Africa"; such "problems" or "issues" of course, never arise when dealing with any other continent.

On the issue of distribution, Lancaster's recitation of authors [namely Cruciani et al. (2004)] who proclaim to have come up short where findings in territories immediately neighboring Ethiopia [like Sudan] is something that deserves closer inspection. Cruciani et al. (2004) themselves don't quite go out on a limb to rule out an "eastern African" origin, but point out two "observations" that point to, in their words, a "Near Eastern" origin:

Although the frequency distribution of E-M34 could suggest that eastern Africa was the place in which the haplogroup arose, two observations point to a Near Eastern origin: (1) Within eastern Africa, the haplogroup appears to be restricted to Ethiopia, since it has not been observed in either neighboring Somalia or Kenya (present study) or Sudan (Underhill et al. 2000).

A number of observations here: Firstly, it is fairly obvious that "eastern Africa" as used here, is a tacit reference to the "sub-Saharan" area of eastern Africa; for if that wasn't the case, then naturally, one would have to assume that Cruciani et al. (2004) are not informed about Egypt being located in "eastern Africa". Moreover, this would be at odds with Mr. Lancaster's "concession". Secondly, the authors here are obviously drawing a far-reaching conclusion from fairly narrow observations made from just two studies that they cite, one of which happens to be their very own—their then present study. The authors study did not include any sample from Sudan, leaving the authors to rely on a single older study.

A comprehensive look back at studies done in Sudan suggest that it is an area understudied, which is interesting, given "Western" academic circles' obsession with eastern African areas; there are very few studies [undertaken by elements of 'Western' academia] that have dealt with that territory, let alone comprehensive region-wide study of Sudan. In any case, if one were to take it as the absolute fact based on a few measly [however instructive] studies undertaken, E-M34 absence or rarity in immediately surrounding [Ethiopia] territories of the likes of Sudan, Somalia and Kenya presents another interesting case.

Like Egypt, territories of Sudan and Somalia are right by the Red Sea, and in many cases, however different the internal clusters, and/or distribution and frequency patterns of markers involved may be, have experienced demic diffusion events involving the same line of clades or macro-haplogroups that now find place on Ethiopian landscape. Ethiopia itself is now essentially a landlocked nation, with the parting away of Eritrea, underlying just how fluid and subjective geopolitical constructs can be, and hence, intellectually not economical to place one's bets [arguments] on purely geopolitical constructs. So, if it were a simple matter of gene flow from the Arabian peninsula or the Levant [seems to be Lancaster's leading impression] of the so-called "Near East", one would think that the probability of E-M34 chromosomes finding their way into Sudan, Somalia, Djibouti, Eritrea, as it has obviously done in Egypt and Ethiopia, should be reasonably high enough.

However, if the mutation itself occurred at some point somewhere in central-eastern Sahara, north of the equator, then it is certainly conceivable a community with a very modest effective population size could have taken a two-pronged dispersal: one in a direction towards the delta region of the Nile River Valley, and the other towards the Ethiopian region. There could have even been an additional one, headed westwards, along coastal north Africa. With such migration, it is not necessary for the migrants to have stayed put in what is now Sudan or say Somalia, and even if they had, negative random genetic drift on settlers forming a community(s) of fairly modest effective population size could have ensured that their numbers in these areas remain at undetectable levels, that is to say, one which could easily evade the foci of the selective and patchy sample undertakings by "Western" academic concerns.

Lancaster made a fuss about paraphyletic E-M34 clades having been detected in the so-called Near East, but not in Ethiopia, based on the few studies that make note of these incidences. As natural, and equally so, he was reminded of the other side of said observations: paraphyletic examples of E-M34 were for instance, noted in a Tunisian sample (Arredi et al. 2004), and an isolated incidence in southern Europe, Bulgarian sample, along with another single one in central Asia (Cruciani et al. 2004), but interestingly enough, not in the so-called "Middle East" either [as per both cited studies used as examples]. Needless to say, it would not make the least sense to pounce on an isolated incidence in a Bulgarian sample or one individual from Central Asia and draw a far-reaching conclusion that this must be the vicinity of where the E-M34 mutation first occurred.

Europe is not exactly the epitome of a comprehensive and wide-variety of distinctive E-M35 clades, and one can make the argument, though to a lesser extent than Europe, that the so-called "Near East" is not a garden of variety either, when it comes to E-M35 chromosomes. Africa, on the other hand, is an entirely different story...naturally. The point being, since it hasn't been phylogenetically demonstrated to date that E-M34 derives from any of the other known E-m35 derivatives in either the so-called Near East or Europe, Africa with its unsurpassed comprehensive range, does not run into that problem.

Furthermore, in many of the areas where E-M34 chromosomes had been detected, as related to Lancaster but not getting through to him, it has been found in tandem with other markers undeniably suggestive of most recent common African ancestry. An example of this is in the Levant, wherein paraphyletic R1 chromosomes [as earlier found in northern Cameroon] where observed in high frequencies in tandem with E-M34 chromosomes in Dead Sea Samples, which interestingly, had far lower Hg J markers than other neighboring so-called "Near Eastern" Semitic-speaking groups; conversely, said Dead Sea sample had much higher incidences of E-M34 than said neighbors, along with the X chromosome G6PD marker, generally stated to be restricted to African samples.

Lancaster somehow managed to mangle up the example, and interpreted it as one that supposedly relies on the notion that if said R1 chromosomes are shared with Africans, then it must necessarily follow that the E-M34 is "African". Continuing with Cruciani et al. (2004),...

(2) E-M34 chromosomes from Ethiopia show lower variances than those from the Near East and appear closely related in the E-M34 network (fig. 2D). If our interpretation is correct, E-M34 chromosomes could have been introduced into Ethiopia from the Near East. The high frequency of E-M34 observed for some of the Ethiopian populations could be the consequence of subsequent genetic drift, which can also explain the lower frequencies (2.3% [Underhill et al. 2000] and 4.0% [Semino et al. 2002]) reported for two large independent samples of Ethiopians.

While Cruciani et al. (2004) claim that the internal variation of Ethiopian showed "lower variances" than those from the so-called Near East, a close look at their own visual aid of the networks in question suggest something different: it shows little distinction between the level of distinctive branching within the Ethiopian index and that of the so-called Near Eastern examples. The distraction that Lancaster offers in reaction to this observation, was simply to query the present author of this blog as to whether he was questioning Cruciani et al.'s genetic credentials. Of course, that very idea is preposterous and immaterial, as a succinct and very specific observation was made about Cruciani et al. (2004) on the diagrammatic interpretation of the respective Ethiopian and "Near Eastern" E-M34 networks, which only needed a direct, and equally succinct and specific rebuttal to the contrary, if the dissident (Lancaster in this case) had one. The diagram in question was this:

Click on the image for better resolution

The subtext accompanied with the diagram reads:
Microsatellite networks of E3b haplogroups. A, E-M35*. B, E-M78. C, E-M81. D, E-M34. Reduced-median and median-joining procedures (Bandelt et al. 1995, 1999) were applied sequentially. A haplogroup-specific weight proportional to the reciprocal of microsatellite variance was used in the construction of the networks. The E-M78 unweighted network (not shown) gave the same quadripartite structure. Unassigned chromosomes (B) showed an intermediate position between clusters α and δ in the unweighted network. Microsatellite haplotypes are represented by circles, with areas proportional to the number of individuals harboring the haplotype. Branch lengths are proportional to the number of one-step mutations separating two haplotypes.

The diagram is there for anyone to examine him/herself, and so, if one wanted to make an argument counter to the one the present author of the blog makes, it should be a fairly straightforward undertaking, without beating around the bush with shallow distractions. The latter part [emphasized in bold] of the aforementioned "second" reasoning provided by Cruciani et al. (2004) just goes to show how much observations made in a particular study are greatly influenced by sampling selections and range by the respective authors of these studies; the differing observations attest to this. If the effect of genetic drift is to be thrown into the mix, as Cruciani et al. (2004) insinuate, then that takes us back to or only reaffirms the point made here earlier, about the effects of that same phenomenon on the demographic history of E-M34 migrants. Interestingly enough, the authors proceed with concluding their segment on E-M34 as follows:

From the Near East, E-M34 chromosomes could also have been introduced into Europe, possibly by Neolithic farmers, but the paucity of E-M34 chromosomes in southeastern Europe (Semino et al. 2004 [in this issue]; present study) weakens this hypothesis. Indeed, as for E-M78δ chromosomes, introduction of E-M34 from Africa directly to southern-central Europe cannot be excluded at the present.

The piece necessitates little further elaboration; it pretty much speaks for itself. The gem in it, of course, is that if E-M34 chromosomes introduced "directly" from Africa to southern Europe is more than probable and explains its distribution there, then why can the same not be true for the so-called "Near East"?

*According to Semino et al.'s report, there is microsatellite indication that much of Ethiopian hg J-M267 examples are of Neolithic provenance...
The majority of J-M267 Y chromosomes harbor the single-banded motif YCAIIa22-YCAIIb22 in the Middle East (>70%) and in North Africa (>90%), whereas this association is much less frequent in Ethiopia and only sporadically found in southern Europe. Considering the distribution of this YCAII single-banded pattern—which, besides the usual stepwise mutational mechanism, could be due to a stable mutational event (one locus deletion or a single-nucleotide mutation in the primer sequence)—we suggest that the motif YCAIIa22-YCAIIb22 potentially characterizes a monophyletic clade of J-M267...According to this interpretation, the first migration, probably in Neolithic times, brought J-M267 to Ethiopia and Europe, whereas a second, more-recent migration diffused the clade harboring the microsatellite motif YCAIIa22-YCAIIb22 in the southern part of the Middle East and in North Africa. In this regard, it is worth noting that the median expansion time of the J-M267-YCAIIa22-YCAIIb22 clade was estimated to be 8.7–4.3 ky, by use of the TD approach (see fig. 4 legend), and that this clade includes the modal haplotype DYS19-14/DYS388-17/DYS390-23/DYS391-11/DYS392-11 of the Galilee (Nebel et al. 2000) and of Moroccan Arabs (Bosch et al. 2001).
If true, this would mean that any "Afro-Asiatic" hg J-M267 from southern Arabia would have had to have been acquainted with the farming subsistence from the Levantine areas, and as such, certain Neolithic social terms associated with such economy would have been available. As noted here before, nothing comes to mind that suggests basic "Near Eastern" or "south Arabian" Neolithic-derived terms in Ethio-Semitic. Kivisild et al. are essentially treating hg J1-M267 as the effective marker for proto-Semitic speakers in Ethiopic populations, as opposed to E-M78 or E-M35 clades, which is odd, because the primary agents of spreading proto-Semitic or proto-Afrisan languages into the "Near East" and the Arabian peninsula in the first place would have largely been E-M35 carriers, who originate from an area where preponderance of evidence—including both genetic particulars and language diversity—places the origin of proto-Afrisan language phylum. This therefore puts hg J carrying groups in the "Near East" and elsewhere on the receiving end of "Afro-Asiatic" language acculturation, not the primary agents of it.

The structuring of hg J clades along linguistic lines within Ethiopian samples simply says that these groups likely merged together from distinctive demographic episodes; one involving the group predominantly comprising of proto-Ethiopic Semitic speakers—likely carrying both hg E-M35 clades and hg J clades—and the other,  predominantly Cushitic speaking groups—that's just about it; it tells us very little about the specific direction from which the aforementioned linguistically-structured markers respectively arrived—be it from the north or from the southern Arabia, short of comprehensive comparative analysis at the molecular levels between the Ethiopic groups and geographically proximate, exotic, non-Ethiopic groups.

Kivisild et al. were compelled to make J1-M267 into THE telltale marker for the spread of proto-Semitic phylum into the African horn, because they recognized the low to absent incidences of E-M78 in south Arabian samples, which no less were not based on actual sampling of Yemeni population at the time of their observations, but at the same time they had to contend with linguistic reconstructions that place Ethio-Semitic languages into the southern branch of the Semitic phylum. For those who are bent on explaining away autochthonous coming about of Semitic languages on African soil, this element entices the cooking up of theories around an origin in the southern tip of the Arabian peninsula, no matter how tenuous.

One cannot also help but arrive at the conclusion that the presence of E-M34 clades in southern Arabia must have been elusive to the authors in question, and that it may well be serving as a marker of Afrisan diffusion into that region. In Cruciani et al.'s 2004 journal, which the authors rely on, not only had E-M34 markers been reported in the sole southern Arabian sample (Omani), but so had the E-M78 counterparts, and they occur in identical incidences. That said, E3b1c1-M34 chromosomes are visibly prevalent in Ethiopian groups, as they are across the whole stretch of northern Africa.

From the earlier mentioned Semino et al. (2004) study, as it concerns the compatibility of Ethiopian J1-M267 clades with the idea of their introduction by southern Arabians, particularly their nearest neighbors—the Yemeni, it was acknowledged that:
The lower internal variance of J-M267 in the Middle East and North Africa, relative to Europe and Ethiopia, is suggestive of two different migrations.  - Semino et al. (2004)
This revelation to the authors' above is consistent with those reported elsewhere, and may well prove instructive in the quest to determine the duplicity of Ethiopian hg J clades with those in southern Arabia, particularly those of Yemen:
At another extreme, the haplogroup distribution of Yemen shows very limited variation, particularly when compared to neighboring populations, Oman and UAE (3 versus 11 haplogroups each), whereas Qatar is intermediate with a total of seven haplogroups, four of which display frequencies of less than 3.0%. Although Qatar does not approximate the lack of diversity seen in Yemen, the two populations display affinities that are apparent in the MDS plot, in which populations of the Levant are interspersed among the South Arabian populations, with Qatar and Yemen segregating apart from both UAE and Oman. - Cadenas et al. (2007)
The authors figure that one of the underlying causes for this reduction in diversity of Yemeni Y-DNA gene pool, in addition to subsequent expansions masking earlier ones, could be a matter of a "high degree of consanguinity" within the population. Having said that, elsewhere, they wrote:
Median BATWING expansion times based on Y-STR data for the Omani (2.3 ky; 95% CI: 0.6–29.2) J1-M267 chromosomes4 indicate a more recent arrival to the South Arabian populations as compared to the older expansion times obtained for the Egyptian (6.4 ky; 95% CI: 0.6–278.5)4 and Turkish (15.4 ky; 95% CI: 0.4–604.8)12 representatives of this haplogroup. Conversely, in the present study, Y-STR age estimates based on the method described by Zhivotovsky et al46 generated much older values for the J1-M267 haplogroup in Yemen, Qatar and UAE (9.7 +/- 2.4, 7.4 +/- 2.3 and 6.4 +/- 1.4 ky, respectively) than seen in the Omani,4 consistent with an earlier arrival to the region during the Neolithic. The data suggest expansion from the north during the Neolithic (or perhaps more recently), which is also reflected in the lower STR variances in southern Arabia (0.14 for Qatar, 0.15 for UAE, 0.20 for Yemen and 0.27 for Oman4 versus 0.31 in Egypt4 and 0.51 in Turkey12). Subsequently, a series of recent demographic events may account for the high haplogroup frequency of J1-M267 in the populations from the present study.  - Cadenas et al. (2007)
Like Semino et al. (2004) and Luis et al. (2004) before them, these authors too note the higher intra-haplogroup variance in J1-M267 chromosomes in areas to the north of the southern Arabian territories mentioned here both in the "Middle East" and Northeastern Africa than those in populations of said southern Arabian peninsula areas. In light of this, it is important to reiterate, just as the said authors themselves did by citing Semino et al. (2004), that the Ethiopian hg J1 clade gene pool was even more varied than those from the "Middle East" and coastal Northern Africa.

Furthermore, just as the aforementioned authors in the 2004 publications note the older expansion ages for populations to the north of the more southward-oriented populations in the Arabian peninsula, the authors here too arrive at the same conclusion, noting that the expansions appear to have began from the Neolithic times onwards from the north to south along the Arabian peninsula. Yet again, Ethiopian hg J clades in turn show older expansion ages than sections of Northern African and "Middle Eastern" gene pool, which have been affected by more recent demographic events, as have the southern Arabian groups mentioned here.

The estimated upper-end expansion time frame of Yemen's hg J1 gene pool in particular is one that is not inconsistent with that generally associated with the spread of Neolithic farming subsistence, but the more internally more varied hg J1 clades of Ethiopia than those in Yemen (not to mention the distinctive aforementioned paraphyletic clades of Ethiopia), and the rest of the south Arabian groups described here, is inconsistent with an idea of introduction from Yemen, or any other southern Arabian territory for that matter. This is significant, considering that hg J has a considerable presence in Yemeni Y-DNA gene pool; 72.6% according to Cadenas et al. (2007).

The picture is no less different, when it comes to E1b1b1c (E-M123 or its subclade "E-M34")  chromosomes; while Cadenas et al. (2007) cited Cruciani et al.'s (2004) reckoning about the plausibility of Ethiopian E3b1c1-M34 bearing chromosomes arriving from the "Near East", they did not go quite as far as suggesting a "Near Eastern" origin for the clade in any context:
On the other hand, Cruciani et al57 have postulated that the E3b1c-M123 clade may have originated in the Near East, as its presence in East Africa is restricted to Ethiopia (11.2%). The median expansion time for M123 in Egypt is 10.8 ky,4 comparable to the estimated age of M123 STR variation obtained through the method described by Zhivotovsky et al46 for UAE (11.1 +/- 3.9 ky) and Yemen (10.6 +/- 4.1 ky), although allelic differences between these two populations indicate that they do not share a common ancestry. Recent archaeological finds supports a trading relationship between Mesopotamia and the Arabian Gulf region dating back to the Al Ubaid Period (~7000 yBP) as evidenced by the excavation of Ubaid pottery from Mesopotamia in UAE.8–10 Ancient maritime trade routes linking Mesopotamia to the Indus Valley included Dilmun (the island of Bahrain) and Magan (in the southeastern tip of the Arabian Peninsula). It is possible that the close ties between Mesopotamia with both the Nile River Valley and the ancient Persian Gulf region during the Neolithic helped disseminate these haplogroups.  - Cadenas et al. (2007)
In fact, the authors' language suggests acknowledgment of E3b1c-M123 as more of a marker of African ancestry than one of "back-migration":
The E3b1-M35 sub-haplogroups, M123 and M78, are believed to have spread from East Africa to North Africa and later expanded eastward through the Levantine corridor and westward to northwestern Africa. Although E3b1a-M78 data suggest that this dispersal occurred in both directions,4,34,47 E3b1c-M123 disseminated primarily to the east.4 The distribution of the E3b1-M35 derivatives in Yemen, Qatar and UAE agrees with their arrival by expansion via the Levantine corridor rather than through the Horn of Africa. This route is similar to general patterns of Levantine mtDNA gene flows during the Upper Paleolithic55 to the Neolithic.5,55 This is immediately apparent by the M35 profile of several East African populations. - Cadenas et al. (2007)
Having noted Cruciani et al.'s (2004) postulation, the authors merely note that the trade network between said regions may have facilitated the spread of E3b1c-M123, which was followed by its expansion downward in the Arabian peninsula, wherein it appears to have undergone multiple founder effect—and likely genetic drift thereafter—events, resulting in the patterns noticed in the Arabian peninsula, with different populations having different subsets of E3b1c1-M34 chromosomes, which of course, brings us right back to Cruciani et al.'s (2004) claim of relatively lower internal variation in the Ethiopian E3b1c1-M34 gene pool than those from the "Near East".

Cruciani et al. (2004) provided us with a map of E3b1c-M123 among other E1b1b1 markers, displaying the Y STR network, wherein they tell us that the Ethiopian examples are more closely related than the "Near Eastern" examples, and hence, lesser diversity thereof. As noted here before, an instant look at the map itself doesn't appear to invoke a sense of that much of a difference between the internal diversity of Ethiopian chromosomes and those of "Near Eastern" examples as far as the number of distinctive inter-connecting "branches" that respective haplotypes fall into is concerned, save to say that where said haplotypes are branched-out immediately from one another and having come from populations within the same region or general geography, the Ethiopian examples do appear to display more shorter-length branches in between them than those from the "Near East". The comparison based on the latter phenomenon though, doesn't come from a level playing field; the Ethiopian haplotypes are expected to show relatively shorter branches with respect to one another, because they are highly geographically-proximate populations of the same nation state, whereas those from the "Near East" were pooled from distinct geographical territories spanning the Asian Minor, the Levant all the way to the southern tip of Arabia.

As we've just seen from above, the "Near East" expansions of E3b1c1-M34, likely first in the northward-oriented territories therein, and then from there towards the south, was marked by multiple "founder effect" situations accompanied by genetic drift—positive or negative, resulting in different subsets of E3b1c-M123 developing internally within respective distinct populations of the region. A fairer assessment would be pooling *all* African examples together, and comparing them with the pooled "Near Eastern" examples. Cadenas et al.'s (2007) posting of internal-diversity of E3b1c1-M34 clades per region is consistent with previous data.

Furthermore, the studies posted here all, and more, keep citing that singular Underhill et al.'s (2004) one study wherein E-M34 was not detected in a Sudanese sample, around which Cruciani et al. (2004) raise their "Near Eastern" origin plausibility, for Ethiopian chromosomes. In that same study, interestingly, no hg J clades were observed in the Sudanese sample either; How is that for a reality check? Yet, other studies have noted hg J markers in Sudanese samples. Semino et al. (2004) for their part, refrained from inferring the origin of Ethiopian E3b1c1-M34 clades, only noting that:
The network of E3b1a-M78 and that of E3b1c-M123 are in agreement with the hypothesis of their ancient presence in the Near East and their subsequent expansion into the southern Balkans. The divergence time (TD) (Zhivotovsky 2001) between the Near East and European lineages has been estimated to a range of 7–14 thousand years (ky) ago. Cinniog˘lu et al. (2004) found a high degree of variance of E3b1c-M123 in Turkey, which has been interpreted as being due to multiple founders rather than a single early dispersal event that has remained geographically circumscribed.  - Semino et al. (2004)
In Turkey alone, we are confronted with a scenario of multiple founder situations; it has implications on the point made herein and that by Cadenas et al. (2007), about this phenomenon having an impact on the pattern of "internal" variation from across a fairly wide region spanning the Asian minor to the southern tip of the Arabian peninsula, when populations therein are pooled together and pitted against that of the more geographically constricted territory of Ethiopia. The high degree of internal variation in Turkey has been attributed to "multiple founders" from different demographic episodes, but Yemeni and other south Arabian populations, as we shall see below [and also revisit info above], display fairly low internal variation for their E-M34 markers, or conversely, high degree of homogeneity...

From Cadenas et al.'s (2007) posting:
As the MDS plot displayed a close affiliation between South Pakistan and North Iran and the former segregated away from the Gulf of Oman populations, the x^2-test was repeated excluding South Pakistan. Although statistically significant differences are still apparent for haplogroup E (x^2 = 10.170, d.f. = 2, P = 0.0062) and R (x^2 = 10.560, d.f. = 2, P = 0.0051), J (x^2 = 2.577, d.f. = 2, P = 0.2757) exhibits an even distribution among Oman, UAE and South Iran. However, a greater homogeneity is observed among the South Arabian populations of Oman, UAE and Qatar for haplogroups E (x^2 = 2.249, d.f. = 2, P = 0.3248), J (x^2 = 4 831, d.f. = 2, P = 0.0893) and R (x^2 = 0.308, d.f. = 2, P = 0.8573). The significant differences in frequency of haplogroups result in detectable clines moving from the South Arabian populations to South Iran and then South Pakistan (E: 18.8, 6.8 and 3.3%; J: 50.4, 35.0 and 25.3%; and R: 11.2, 25.6 and 46.2% for South Arabia, South Iran14 and South Pakistan,30 respectively).  - Cadenas et a.l. (2007)
The above show gradients, wherein hg E is greatest in southern Arabia and decreases as one proceeds northward to southern Iran, and then eastward to south Pakistan, and the same applies to haplogroup J, while the reverse trend is seen in hg R, with the lowest frequencies in southern Arabia. The piece above notes a greater loss of diversity in all three haplogroups involved when it comes to southern Arabia, compared to the other mentioned regions. Yemeni gene pool for these are yet even more limited in their diversity than the aforementioned south Arabian populations of Oman, UAE and Qatar. Recalling the piece cited earlier:
At another extreme, the haplogroup distribution of Yemen shows very limited variation, particularly when compared to neighboring populations, Oman and UAE (3 versus 11 haplogroups each), whereas Qatar is intermediate with a total of seven haplogroups, four of which display frequencies of less than 3.0%. Although Qatar does not approximate the lack of diversity seen in Yemen, the two populations display affinities that are apparent in the MDS plot, in which populations of the Levant are interspersed among the South Arabian populations, with Qatar and Yemen segregating apart from both UAE and Oman. - Cadenas et al. (2007)
Luis et al. (2004) posted an internal variance of  .41 for the Egyptian sample vs. the just .05 internal variance for the Omani sample, as it pertains to the E3b1c-M123 clade. Likewise, the internal variation of Egyptian J clades—J-12f2(xJ2-M172) and J*-12f2(xJ2-M172)—was .45 and .31 respectively, while those reported for the Omani sample were .40 and .27 respectively. There is apparently greater disparity between the reported values for the two samples in the case of the E-M123 marker than the J clades, but the common element here is the relative greater internal variation in the Egyptian sample vs. the Omani. On the other hand, Cadenas et al. (2007) report the following internal variance values for the following groups respectively: For UAE the value was .25 [E3b1c-M123] and .15 [ J1-M267], while for Yemeni, the values were .14 [E3b1c-M123] and .20 [J1-M267]. Qatar did not report for any E3b1c clades, but did have a value of .14 for J1-M267. The level of diversity demonstrated above in eastern African examples of E3b1c-M123 and J1-M267 markers, including those from Ethiopia, is inconsistent with a south Arabian origin. 

The plausible scenario for introduction of hg J1 clades in Ethiopian populations is more likely one wherein the J1 clades arrived early in the continent, whether due to in situ origin or back-migration, and was picked up by the ancestors of contemporary Semitic speaking groups of Ethiopia somewhere in the Sahara, noticeably more northward than their current habitat. However, even if one were to entertain a back-migration scenario as the causal factor for all African J1 clades for arguments sake, it appears to have arrived when the clades were in their very early stages of diversification, hence the abundance of paraphyletic clades, as discussed elsewhere here [link].

If the spread of the Neolithic farming subsistence played any role in all of this, then one would have to contend that it likely did not accompany some massive movement of people from the so-called "Near East", but rather, small scale migrations likely induced by increasing social organizations in both the so-called "Near East" and the Sahara, paving way to the opening of early and perhaps modest trade routes. This may have primarily involved fauna and possibly flora in the Neolithic context, between populations in each area with a view to liven up their preexisting stock with more choices or variety [with 'exotic' goods]. As such, would-be migrants who decided to settle in a new homeland, simply integrated into preexisting Saharan communities, and thereby not shifting the traditions of host communities in any considerable way.

We've already gone through the lack of immediacy between the so-called "Near Eastern" Neolithic farming "revolution" of economy therein, which is significant, considering that "Near Easterners" are right next door to the northeastern corner of Africa—one would expect entry into the Nile Valley through the Sinai corridor to have been more immediately accessible than many of the areas in mainland Europe, and hence, serve as one of the earliest entry points of demic-diffusion accompanying the spread of a tradition largely of a farming subsistence economy; several researchers claim that archaeological indicators suggest that large farming in northeastern Africa came in more or less about the same time as its spread into some areas in northern Europe, between ca. 8ky to 6ky ago or so, which makes it either on par and/or even later than certain southern areas of Europe. Yet, conversely, cattle domestication is said to have likely arrived before or about the same time as that in the so-called "Near East".

These developments don't square with massive intrusion of migrants into an area, thereby considerably shifting traditions in the destination point. It is against this backdrop that no considerable language shifts would have taken place either, which makes more sense in the Ethiopian context, considering the lack of tracing of "Near Eastern Neolithic-derived" terms in Ethio-Semitic languages. Now of course, the alternative to all this, is that hg J1 could have originated in the Sahara near the northeastern end of the African continent, and it certainly cannot be ruled out in the final analysis—just as the aforementioned matter of great internal diversity and paraphyletic clades demonstrate, but this is a matter that understandably doesn't sit well with many "westerners", just as the fact that E3b1a-M78 could be considered African ancestry [which if any, is usually begrudgingly accepted as African, preferably "northeast as opposed to sub-Saharan African"] and seeing as how both it and the J clade are widely acknowledged to be important markers of the diffusion of the so-called Neolithic farming subsistence into Europe.*

The argument revolving around the "Semitic" language family carries very little weight in the big scheme of things; it appears to be one that Lancaster heavily relies on. The Semitic language branch is after all, merely an offshoot of a language family that originated and spent the bulk of its evolution in Africa before spilling over to nearby territories.

Ethiopia has a distinction of being the most concentrated area for a fairly wide variety of a Semitic branch, which in this case, is what's deemed within "Western" academic circles as the south Semitic branch. When pressed on any justification for ruling out a possible direct African origin for the Semitic subphylum, Lancaster offered no specific or substantive explanation, other than to simply generalize that certain segments of "Western" academia profess a "Near Eastern" origin for the Semitic offshoot of the African language superphylum.

It is of note, that for all such speculation that Lancaster takes for granted as gospel truth, not one of these proponents have effectively demonstrated how [lexically and grammatically] and in what specific time frames Ethio-Semitic branch purportedly derived from the South Arabian branch. Sure, there are deemed to be some similarities here and there, which should be expected, given the history of bidirectional contact across the Red Sea, but this is far from establishing the particulars just mentioned, regarding time and nature of a purported derivation. Time can for example, be extrapolated from key root terms, with the aid of archaeology and other disciplines like bio-anthropology; examples of the sort, include proclaimed "Neolithic" terms. None has been identified, in the case of connections between Ethio-Semitic and known South Arabian dialects. As matter of example,

Andrew Lancaster writes:

But Lionel Bender (1997), a leading expert on Ethiopian languages, proposed a scenario upon linguistic grounds wherein Semitic languages originated in Ethiopia and crossed the Red Sea. We can note that although this linguistic theory would be in line with these very particular and unsurprising genetic links between the Horn of Africa and the Southern Arabian Peninsula, it does not correspond to much else in genetics or archaeology, and there is no reason to invoke such a theory in order to explain genetic links between the Horn of Africa and nearby Southern Arabia.

While Lancaster in his own little way seeks to downplay Bender's take on the geographic origin of Semitic, which in his very own words was proposed "upon linguistic grounds", by way of thereafter making references to its misplaced impact or implications on molecular genetics and archaeology that no specific personality had made in the first place, he provides no answers to the requested linguistic particulars just discussed above. His need to invoke Bender here, is to presumably discourage potential usage of Bender's proposal for the very thing that he admits is the case:

We can note that although this linguistic theory would be in line with these very particular and unsurprising genetic links between the Horn of Africa and the Southern Arabian Peninsula.

His real concern here, is that Bender's findings may be seized upon as yet another line of evidence that can be used to buttress an "African" origin point for E-M34, and its subsequent spilling over to nearby territories, as opposed to vice versa. That said, Lancaster's line of defense for not being able to substantively explain the nature and time-line of the genesis of a "Near Eastern" origin for the Semitic subphylum, culminating in Ethio-Semitic in the African Horn, is that he is not a professional linguist and has to rely on others; any clear-headed individual can see right through it as a dismissive line of defense, because if one is bold enough to take sides, then one ought to know the particulars of the arguments they are siding with. When pressed on it, Lancaster was not even able to offer further explanation for the immediately obvious loose ends that Chris Ehret et al's (2009) recent paper seem to be suffering from. The paper in question was: Bayesian phylogenetic analysis of Semitic languages identifies an Early Bronze Age origin of Semitic in the Near East, which itself is a noticeable departure from Ehret's own past viewpoints on the genesis of Semitic. Change of viewpoints is of course not unusual in academia, but it has to be accompanied with a well-defined intellectual justification, that is clearly laid out before the audience. The abstract put forward for the just-now-mentioned paper goes like this:

Kitchen A, Ehret C, Assefa S, Mulligan CJ. Department of Anthropology, PO Box 103610, University of Florida, Gainesville, FL 32610-3610, USA.

The evolution of languages provides a unique opportunity to study human population history. The origin of Semitic and the nature of dispersals by Semitic-speaking populations are of great importance to our understanding of the ancient history of the Middle East and Horn of Africa. Semitic populations are associated with the oldest written languages and urban civilizations in the region, which gave rise to some of the world's first major religious and literary traditions. In this study, we employ Bayesian computational phylogenetic techniques recently developed in evolutionary biology to analyse Semitic lexical data by modelling language evolution and explicitly testing alternative hypotheses of Semitic history. We implement a relaxed linguistic clock to date language divergences and use epigraphic evidence for the sampling dates of extinct Semitic languages to calibrate the rate of language evolution. Our statistical tests of alternative Semitic histories support an initial divergence of Akkadian from ancestral Semitic over competing hypotheses (e.g. an African origin of Semitic). We estimate an Early Bronze Age origin for Semitic approximately 5750 years ago in the Levant, and further propose that contemporary Ethiosemitic languages of Africa reflect a single introduction of early Ethiosemitic from southern Arabia approximately 2800 years ago. - abstract ends -

Supposing one were entertaining the authors' proposal, the 800 BC or so date, or even if one were to extend this date to the beginning of the south Arabian influences coinciding with the emergence of the the D'mt complex, logically suggests that any already differentiated and fully developed south Arabian "Semitic" language that diffused into the African Horn would have been adopted as is, meaning—in the very shape or form the language was brought in and expected to be no different, especially in the era it was introduced. However, from archaeology, and citing personal notes posted elsewhere:

"The inscriptions dating from this period in Ethiopia are apparently written in two languages, pure Sabaean and another language with certain aspects found later in Ge`ez (Schneider 1976). All the royal inscriptions are in this second, presumably Ethiopian, language." - Stuart Munro-Hay

What does this imply? "Pure" as used here, suggests that although "Epigraphic South Arabian" alphabets were used to convey a message in two different languages [one for south Arabian administrative centers, and the other for the comprehension of the locals (aka "Ethiopian" people, i.e. Eritreans, Tigrinya or what have you)], one of the languages on the inscriptions was the south Arabian language that was brought in along with South Arabian immigrants, while the other was a local language aka a local "Ethiopian" language.

Though both languages were written in ESA alphabets, evidence above suggests that the "Ethiopic" language very likely had grammatical features that clearly distinguished it from its Sabean/south Arabian counterpart. The aforementioned citation of Munro-Hay should be instructive, once again:

"...and another language with certain aspects found **later** in Ge`ez (Schneider 1976).

Now, Ge'ez is considered to be Semitic, which therefore follows that this ancestral language was the proto-Semitic language of Ge'ez.

Also, the era suggested in the abstract implies that the Neolithic J carriers of the region [Ethiopia, presumably migrants originally from South Arabia] didn't already speak some form of proto-Semitic or Semitic, but rather, that this only came into being around the time of the D'mt complex, give or take.

Now of course, the authors of the study at hand could attempt to move their dates and make it coincidental with these Neolithic era groups, but they would have to come up with a good deal of "south-Arabian" imported Neolithic root terms for the Ethio-Semitic branch, which hasn't been produced to date, that comes to attention, especially given that the Neolithic in the African Horn has been more linked to those of the Nile Valley in the Sudanese region, in terms of influences, than those in the Levant or south Arabia as sources of inspiration.

Furthermore, we are told:

"another language with certain aspects found later in Ge`ez (Schneider 1976). All the royal inscriptions are in this second, presumably Ethiopian, language." - Stuart Munro-Hay

The emphasized bit goes right back to the following, as already stated herein:

[One for south Arabian administrative centers, and the other for the comprehension of the locals (aka "Ethiopian" people, i.e. Eritreans, Tigrinya or what have you)], one of the languages on the inscriptions was the south Arabian language that was brought in along with South Arabian immigrants, while the other was a local language aka a local "Ethiopian" language.

If an observer takes issue with assigning "African origin" to E-M34 even as he/she professes to concede to an "Egyptian origin", then they are most surely suffering from the same psychologically-seated self-contradictions as Mr. Lancaster. It found expression in his debate etiquette, which visibly devolved for the worse, bordering juvenile at times as he sought to threw in off-topic distractions and playing the role of multiple personalities—doubling as a regular poster under his regular or actual name, and suspiciously, as "a moderator" with a pseudonym who only appears when the heat is being turned on Lancaster, as his viewpoint is put through its paces. It is not that Lancaster does not see a very compelling argument for E-M34 as essentially an African marker or lineage, it is just that he stigmatizes "Africa" and turns anything rightfully associated with it into something offensive [to him]. Presents a dilemma? Far from it; an African origin of E-M34 is more than compelling!

As regularly advised on this site, lookout for future updates.
_________________________________________________________
References*

— As noted in the body of the passages above.

— The passage placed in between two ' * ' signs was borrowed from a later posting [link] than the present one, because of its relevance to the discussion at hand.

—Semino et al. (2004), Origin, diffusion, and differentiation of Y-chromosome haplogroups E and J: inferences on the neolithization of Europe and later migratory events in the Mediterranean area.

—Cruciani et al. (2004), Phylogeographic Analysis of Haplogroup E3b (E-M215) Y Chromosomes Reveals Multiple Migratory Events Within and Out Of Africa.

—J.R. Luis et al. (2004), The Levant versus the Horn of Africa: Evidence for Bidirectional Corridors of Human Migrations.

—Cadenas et al. (2007), Y-chromosome diversity characterizes the Gulf of Oman.

—*Personal notes taken from notes dated to 2009 and 2010.

Saturday, August 15, 2009

Who were the Ancient Israelites? A Multidisciplinary search for Answers

Image Caption: "Parting of the Red Sea", during the alleged emigration of ancestors of Jewish people, made famous by the biblical legend. Click on the image for enlargement
Introduction: 

 

Over the years, this has become an age-old question: Who were the ancient Israelites, really? In what capacity did they actually exist, or what not, and do biblical stories around their eponymous ancestors and rulers reflect actual events?

This is actually a topic that came to attention quite a few years back, and to this day, the topic is controversial as ever. Part of the reason it is controversial, is the religious loyalties of elements both within and outside academia, that said persons want to defend. The other, which like the just-mentioned, probably goes without saying, is apparently longstanding identity/racial politics which certain camps are intent on passionately defending.

On the academic front, say for example—Egyptology, those who do not wish to shake up long held traditional narratives—usually highly romantic portrayals that have become cemented in many minds, with the aid of the likes of Hollywood—of what went down in ancient Egypt (Kmtnwt), and are perhaps in part inspired by biblical narratives, are also very reluctant to look at the subject at different, perhaps refreshing and more sober, angles. Whatever the camp, these ideologues generally share in common, resistance to re-examining their prevailing perceptions/preconceptions in the face of ongoing inflow of strongly substantive material, which challenge said viewpoints.

Friday, July 31, 2009

Striving for Clarity and Accuracy

There are various aspects of academia that some of us take for granted, without critical thinking. The driving "wisdom" here, is that if an idea is an oft repeated or recited one in sections of academia, then it must certainly be accurate and doesn't call for further investigation. This almost certainly appears to have been the case in a recent personal encounter with a question dealing with "bovine" taxonomy, one concerning the Fulani cattle in particular. It has generally been the case in "western" academic circles, to casually refer to Fulani cattle breeds as "West African Zebu", and as such, one opinion encountered fairly recently in an internet discussion forum, figured that such a description is fine and dandy, because Zebu happens to be accompanied by the "West African" descriptive, thereby invoking the subspecies' precise African origin. That may well be so, but does that at any rate, necessarily render the Zebu-characterization correct? After all, here, it is not simply the matter of the cattle subspecies' geographical origin, but its actual genealogical heritage. As far as the latter goes, i.e. the genealogical question, this was greeted with an explaining-away that "implicitly" argues that the West African "Zebu" makes sense given the relatively predominant Zebu genetic contribution over those of other bovine lineages [namely the Bos Taurus — either African and/or European] in the West African "Zebu" gene pool. To this end, sources such as that compiled by E. M. Ibeagha-Awemu. et al. 2004 have been offered as evidence; it goes like this:


Click on the image for hi resolution

The argument given based off this piece of information, as it goes, appears to be that most of the Bovine types sampled herein are "hybrid", because of their apparent "inter-subspecies" ancestries, and that notwithstanding, has no bearing on their assigned taxonomic identity; the unspoken "wisdom" here, is that since this piece of information appears to suggest that most cattle breeds out there are rarely "pure", and hence are inclined to be "hybrid", then the condition of having ancestry from divergent subspecies doesn't have any bearing on a cattle breed being assigned to only one of the subspecies. Instead, contribution from the other parental subspecies — whose taxonomic identity had not been assigned to the offspring — is interpreted here as "admixture". The tacit premise—wittingly or unwittingly—that this logic lies on, is that the breeds are assigned their taxonomic identity based on which parental type's contribution to their gene pool supposedly 'predominates' or 'prevails' over others'. Indeed, just reading off of that table above, for example, one comes out with the understanding that many of the African breeds assigned the "Zebu" descriptive appear to have more "Zebu" contribution than their Bos Taurus counterparts. However, further investigation involving other set of markers cautions that the situation is more complex than that imagery.

You see, one has to bear in mind, that we are dealing with domesticated fauna here, which means that whether or not the herders are actively aware of the precise set of phenomena that impart advantageous features to their livestock, awareness of a trend(s) in the success of a breed will lead to every effort [by the herders] at sustaining or maintaining, if not swelling the populations of said breed. The type of markers implicated in the table above, do not necessarily allow one to adjudge the significance or precise magnitude of contribution from the parental inter-subspecies elements involved; and why is that? Because again, the Fulani was the product of domestication, meaning that it was intentionally crossbred to bring out and retain certain advantageous features from the contributing parental bovine subspecies types. These advantageous features would have had to have been encoded in certain DNA nucleotides, rendering certain DNA loci under "selection pressure". Such a situation would therefore make these loci insufficient in ascertaining the actual level of respective contribution from the contributing parental bovine "inter-subspecies" involved. Other loci may well simply be relatively stable in the face of random genetic drift, by chance occurrence. For instance, a largely "hybrid" population comprising Zebu-Taurine individuals might not accurately reflect contribution of the "parental" subspecies involved, because certain loci could, by chance occurrence meted out by "positive" random genetic drift, remain relatively stable in their distribution across the population, simply because the number of "hybrid" individuals which just-so-happen to be homozygous at the locus in question happens to be the relatively overrepresented one than that comprising individuals which are heterozygous at said locus. To demonstrate how all this could be, one only need to look at what uniparental genotyping from the so-called African Zebus thus far reveal: these tests show that in the case of West African "Zebu" breeds, a majority of them have "Zebu" paternal ancestry, but by contrast and almost "exclusively", they have the Bos Taurus maternal ancestry! This is a true definition of a "hybrid" ancestry. It is not as if the West African "Zebu" are largely "Zebu" in both maternal and paternal ancestry, and that only a small segment of their population is "Zebu" and "Bos Taurus" in ancestry, so as to deem the "aberration" as "admixture", but that this is true for virtually all the African "Zebu", that they happen to have both "Zebu (Bos Indicus)" and "Bos Taurus" uniparental ancestry.

Yes, there are hybrids amongst the so-called African breeds of "Bos Taurus" as well, but unlike their so-called "Zebu" counterparts, uniparental genetyping show that there are actually sizable populations of these "Bos Taurus" who have virtually ONLY "Bos Taurus" ancestry both maternally and paternally.

The argument that many herds are likely to have "inter-subspecies" ancestry, and therefore are 'hybrid' and the condition has no bearing on their assigning to one or the other taxonomic [bovine] group, is immaterial to the fact that genealogical and archeological particulars point an "independent" African Bos Taurus domestication, and hence, considered a "true" phylogenetic entity on its own that distinguishes it from the "Near Eastern" or European Bos Taurus and Bos Indicus. Nor is it necessarily the case, as uniparental genotyping attest to, that African Bos Taurus populations are inclined to be breeds of "Taurine-Bos Indicus" lineage; there are considerable populations of African Bos Taurus, particularly in Western Africa, that are still virtually all Bos Taurus in their lineage! This however, as just demonstrated, is not the case with the western African "Zebu" breeds like the Fulani. The following piece, from Blench et al.'s compilation, gives us an illustration of what has just been described, in relation to uniparental genotyping:

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And...

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Click on the images for better resolution

The other argument made, in addition to the genealogical one just examined, goes like this:

They are zebu, African zebu, as their physical characteristics and their genetics make clear.

Indeed, the present author of this blog is aware of the naming schemes based primarily on morphological traits; that though, says little in actual genetic basis for such terminology. In fact, many of the so-called West African "Zebu" breeds appear to bear traits that are considered "intermediate" in relation to Asian Zebu breeds. This includes things like for example, the less prominent humps on many of the West African "Zebu" breeds when compared to those of their Asian counterparts. It has even led to coining of such terms like "Zeboid"; see, courtesy of Blench et al.'s compilation:

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Click on the images for better resolution

Generally, the bovine subspecies have become casually synonymous in dichotomous terms, with hump-less breeds being synonymous with "Bos Taurus" or Taurine descriptive and humped subspecies being synonymous with the "Zebu" descriptive; this, usually regardless of actual genealogical particulars.

The last line of defense for upholding the viewpoints examined and rebuked [or at least challenged] herein, about the justified assigning of West African "Taurine-Bos Indicus" hybrid breeds like the Fulani to just the Bos Indicus taxonomic group, appears to one of logical fallacy 'appeals to popularity'; that is to say, that many in "western" academia circles simply refer to Western African breeds like Fulani as West African "Zebu", and therefore they must necessarily know all and have the last word on the subject matter. Any challenge to this viewpoint or call for further investigation into the subject matter, to iron out potential incoherencies, peculiarities or inadequacies is dismissed out of hand as emotionalism or an attempt at making an argument simply for argument's sake. It matters not, the fact that all the sources cited by its defender (advocate) too agree with the position maintained by the present author of this blog, as well as "materially"-supported [as the plentiful citations herein bespeak] by the present author, that the so-called West African "Zebu" are IN FACT "hybrid" breeds of BOTH "Bos Taurus" and "Bos Indicus", and so from that fact alone, it would actually be inadequate and inaccurate to deem these hybrid breeds as simply "Zebu". Superficial reasonings aside, the taxonomic assignment as such doesn't really reflect the genetic reality at hand, other than its ardent defenders crying that the Zebu do in fact form part of the genealogy; but still the keyword here, is "part"! A defender of the said taxonomic assignment even went as far as using human socio-constructs as the presumably perfect analogy of the situation at hand, pointing out that despite "hybrid" ancestry, humans are inclined to assign themselves to just one "ethnic"/"racial" group or the other. If said defender were not blindsided by ideology, it would have occurred to the person that not only are the socio-contructs in question not regarded as scientific, but also humanity from a biological standpoint, does not comprise of several distinct "sub-species"; our variations have not been that significant to warrant division of humanity to several distinct subspecies. Such is the sort of weight [as it relates to substance] with which the layperson approaches questions relating to the subject matter of this very blog topic. Is it possible that better explanation is afforded by the more informed defenders, lettered in the disciplines of bioanthropology and molecular genetics, than those examined herein? Perhaps, but from accessible material spread over the net and libraries, none has come to attention that adequately and accurately justifies the taxonomic assignment of the West African Bos Indicus-Taurine 'hybrids' to just the "Zebu" phylogen. The quest for accuracy and clarity should not be dismissed as an abomination, but as an opportunity for furthering knowledge, and the specifics demonstrated here thus far go to show just why!

*As always: Lookout for ongoing updates; notes on this site are regularly updated as information comes to attention!

Sunday, July 12, 2009

Nehesu: What does It Mean?

For those who have ever been part of a Nile Valley discussion, the word "Nehesu" is a familiar one. However, this word seems to be shrouded in a bit of mystery, as far as its precise meaning is concerned. One thing is certain about it, in that it was generally referenced towards groups beyond Kmtnwt's (Dynastic Egypt) southern boarder, particularly beyond where lower Ta Seti lay. Several guesswork have taken place over the years, about its most purportedly-plausible meanings; however, these appear to be just that — guesswork. This includes the reported Budge translation of the term to "Negroes" , and other translation efforts which place the word as a term supposedly commonly associated with "slaves", "Negro" slaves. See for example, the following pieces:







Click on the images for hi resolution.

Speaking of which, some personal notes of the present author [of the blog] were made on discussion boards a few years back, mid-2005 to be precise:

Nhsw
Various folks connect it with "race", in particular "black" or "negro". On that note, it appears that there were/are proponents out there, however very few, who have tried to associate the term with some sort of a "hamitic" race, or what is supposedly "non-Negroid" black folks. As such, the word "nuhas", which denotes "copper", has been used to hypothesize that, the Nehesu were likely named after their “copper toned skin”, and in the terms of these advocates, this would mean "copper skin caucasoids"; not much different from how some folks here refer to “brown”, as a way to runaway from having to say ‘black’.

To my understanding, this word has been documented in the Old Kingdom, and it appeared that early Egyptian artwork used it in connection with "southerners", who were painted in the same brown skin tones as Kemetians. But then, in the Middle, Second Intermediate and New Kingdoms, it was applied to "southerners", who were depicted in varying colors from reddish brown to pitch black, including various dress styles, hair styles or wigs, and varying facial features. Aside from the “southerners”, I am not aware of the application of “Nhsw” to any other people.


Again, turning to languages still spoken in the region, like the Beja, various words have been utilized in hypothesizing possible origins of words, and their meanings. As an example, a Beja word “nehas”, which is understood as ‘being pure”, has been used in this manner. There is at least one perspective of connecting the word with a possible Kemetian viewpoint of their southern roots, i.e., their “pure” ancestors [pure, as in less foreign admixture], whereby this was looked at in relative terms, not absolute.


At the time these notes were presented, one opinion on the matter went like this, by a third party observer:

bonjour,

I think Nhsy's were a tribe or group of people linked together by rituals different from other parts of "deeper" Africa, who derived ntrws to animalism, totetism, etc….. whereas the Nhsy were praising a lot and praying, could be circumcising their children, because of the hieroglyphic spelling of the name, if you decorticate, you will have in prayer, n-Hst, hsi to praise,n-hsy or the ones in praising just my take on this.


If you look at the glyphs, you will see why i feel that way, the rope the walking stick, (moving tribes materials) and the rebel sign, the man with his hands behind his back, no! if i make any connection to Moses i will be fired.
but then i may be complètement à côté de la plaque.

sw

Of course then, the feedback offered by the present author was to the effect of:

I've heard this one too, along the lines of, 'being praised by God or a god', or 'the chosen people'...by God. This is supposed to be what the 'hsy' or 'hsw' in 'Nhsw' signifies, from this viewpoint.

But getting back to the glyphs presented above, at least one translation given to a variant of "Nehesu" was "peasant" — that Mdu Ntr term in particular, was "Nehi"; see the glyphs again:


Click for hi resolution

Along those lines, an effort was made by a third party advocate to tie "Nehesu" with a string of distinct nouns generally of a 'pejorative' connotation...


Click for hi resolution

The above was for instance, described and translated in the following manner:

...and this was a name of a 13th Dynasty Pharaoh .

Note that his Nsu Biti title contains the determinative for "Barbarian" - his SaRe title merely lists him as "Nahasi" - which I interpret as "The Barbarian" - a synonym in Mtau Ntr for "Peasant, supplicant, worthless ..."

The Greeks would later borrow this ideological terminolgy: Barbarian, for any people who were not Greeks...

The translation no doubt raises a red flag instantly, if for nothing else than the odds of a King, a figure who is generally quite protective of or guarded and sensitive about his image both during life and after death, allowing himself to be identified by anything that is synonymous with "worthless", unless of course, it was a case of some sort of "commemorative" gesture of ridicule bestowed upon him by a successor who despised him and sought to have history remember him in a soiled or less-than-flattering image?

While these various above-mentioned translations — bearing either pejorative connotations to them or else less-than-flattering air to them — were being offered, another set of meanings was being simultaneously offered for "Nehesu (Nehesw)" by the said third party, hence presenting sets of lexicons at odds with one another, at least in so far as conventional thinking goes; see for example, allegedly based off material cited from a Budge authored dictionary:

Nhsj (Nahasi)- a Sudanese man
Nhsj.t (Nahasit) - a Sudanese woman
Nhsjw (Nahasou)- Sudanese; southerners in general
Nhsjw (Nahasou)- The Sudanese tribes in the Tuat, the results of the masturbation of Ra.

The way it's written

Most words in the Mdw Ntr that began with the letter "n" were written with the hieroglyph of a water ripple; the word "Nhsjw" was written using the 'Guinea-fowl' glyph with the pronunciation being "Nh;Neh;Nah" - very few words began with this 'letter.'

...

In Budge's dictionary, alongside the word "Nehsi" there are additional hieroglyphics showing the different ways of writing the "Nehsi" in the plural. What interests us here is the following example:

Hieroglyphic:
(throw stick)+ s + (sedge plant) + "ou"

--The "throw stick" can be either a determinative; an ideogram; or a phonogram "rs" or "Aa"; at the beginning of a sentence it's a phonogram (and probably also an ideogram which indicates "foreign".)

--The "sedge" hieroglyph ("(n)su; sut") indicates the south; ie;Upper Egypt, Sudan...
So we have "rs"+ "s" + "su" or "Resou" which means "southerners" with the use of the "throw stick" to indicate "foreign southerners"; the same as it is used in the word "Aamu" to indicate "(foreigners)Asiatics"...

:essentially all non-Egyptian peoples in the south; "strangers"

......................


There's one Pharaoh that I came across while reading "Egyptian Language: Easy Lessons in Egyptian Hieroglyphics by EWB". If I recall correctly, the example text was from the "Stelae of Pa-Nahesi" (25th Dynasty?).

I personally think that the title "Pa Nahasi" was a self-deprecating play on words that would imply something like the Pharaoh calling himself "The Barbarian!" or "The Stranger!" - literally it means "The Sudani man". (not "The Nubian")

At the very least, these highly divergent translations of the term underlie the highly speculative nature of the translation efforts that have approached the term over the years.


Click for hi resolution

Furthermore, in reference to the cartouche [reposted immediately above] allegedly bearing a pharoah's name, which has "Nahasi" in it, it is simplistic to take "Nehesw" as "stranger", for if that were the case, there would be no need for a determinative/ideogram suggestive of "foreigner" in say, the first cartouche. That determinative is there, precisely to modify the meaning of the noun in question.

And as noted a few notes ago, one of the reasons for reposting extracts from an old internet boardroom discussion, "Nehesw" seemed particularly reserved for groups from beyond Kemet's (Dynastic Egypt) southern border; it wasn't applied to those in Kmtnwt's east, north or west, as far as recollection allows. Thus 'Nehesw' has got to be more than a synonym for "stranger", or its Greek equivalent of "barbarian" which is not reserved for any one group or territory, as the former appears to be. The precise meaning of the term continues to be uncertain, but what is clear in the meantime, is that neither "negro", "worthless", "peasant", "stranger", "Nubian" which is a Roman corruption of a Nile Valley term that was used in markedly different context from that of the Romans and their 'contemporary' European imitators of the word, nor "Sudan" — which is a fairly modern construct, cut it, as far as logical interpretation goes for reasons gone over. As such, this leaves a space here that calls for watching-out for future developments, in both the not-so-distant and distant future!

Sunday, June 21, 2009

Reviewing: The Genetic Structure and History of Africans and African Americans

This is a carryover from the "miscellaneous notes" of the last blog posting (The Creation of a Fake Controversy: The Fula Origins). It pertains to extracts taken from: The Genetic Structure and History of Africans and African Americans, a publication by Tishkoff et al (2009). that just came out recently. Here, we will examine the work a bit more:

Sampling entailed...

We studied 121 African populations, four African American populations, and 60 non-African populations for patterns of variation at 1327 nuclear microsatellite and insertion/deletion markers. We identified 14 ancestral population clusters in Africa that correlate with self-described ethnicity and shared cultural and/or linguistic properties.

And mentions of interest here...

Ethnic groups, sample size, language classification, and subsistence classification are given in Table S1. For the Kenyan, Sudanese, Nigerian, and Cameroonian samples, white cells were isolated in the field from whole blood with a salting out procedure modified from (S1) and DNA was extracted in the lab with a Purgene™ DNA extraction kit (Gentra Systems Inc., Minneapolis, MN). Dogon samples were obtained from blood spots donated by participants in a cohort study of malaria incidence in Bandiagara, Mali. Ghanaian DNA was extracted onsite from whole blood, with the Purgene™ DNA extraction kit. The Juspeaking !Xun (a.k.a.Vasekela) and Khoe-speaking Khwe samples were collected from individuals in the area of Schmidtsdrift in the North-West Cape of South Africa. The Cape Mixed Ancestry (CMA) population, commonly referred to as Cape Coloured in South Africa, was collected in the Western Cape Province. The Dogon sample was collected in Bandiagara, Mali. Nigerian samples were collected in Abuja and Adamawa State districts. Cameroon samples were collected from the Eastern Province (Baka Pygmies and neighboring Bantu groups), Southern and Ocean Provinces (Bakola Pygmies and neighboring Bantu groups and coastal groups), Center Provinces (Medzan Pygmies and neighboring groups, mostly Bantu populations), Western Province (Bamileke and Mbororo Fulani groups), Extreme North Province (Mandara mountains and northern plains; Fulani and Afroasiatic/Nilo-Saharan speaking populations). All Cameroonians were sampled in their native village; the Hausa sample (a population who emigrated mainly from the Kano area two generations ago) was sampled in the city of Yaounde. Samples from Chad, CAR, Congo, DRC and Rwanda were obtained from individuals who recently immigrated to Cameroon. Tanzanian DNA samples were collected from individuals residing in the Arusha and Dodoma provinces of Tanzania. Samples from Kenyan populations of southern Ethiopian origin (Burji, Konso) samples were collected in the Rift valley, Nyanza, and Eastern provinces of Kenya. Sudanese samples were collected in the Khartoum and Kasala provinces of the Sudan. The Yemenite Temani and Ethiopian Beta Israel samples were purchased from the National Laboratory for the Genetics of Israeli Populations (S2) . The South African !Xun/Khoe, Xhosa, Vende, Cape Mixed Ancestry (CMA), Yemenite Temani, Beta Israel, and the Malian Dogon DNA samples were amplified by Whole Genome Amplification (WGA) with Illustra GenomiPhi HY™ kits provided by GE-Healthcare (Buckinghamshire, UK). It should be noted that the DNA for the Dogon population extracted from blood spots appeared to be of lower quality and microsatellite markers did not amplify as well as other samples obtained from whole blood (43% of markers had missing data).


Abstract:

Africa is the source of all modern humans, but characterization of genetic variation and of relationships among populations across the continent has been enigmatic. We studied 121 African populations, four African American populations, and 60 non-African populations for patterns of variation at 1327 nuclear microsatellite and insertion/deletion markers. We identified 14 ancestral population clusters in Africa that correlate with self-described ethnicity and shared cultural and/or linguistic properties. We observed high levels of mixed ancestry in most populations, reflecting historical migration events across the continent. Our data also provide evidence for shared ancestry among geographically diverse hunter-gatherer populations (Khoesan speakers and Pygmies). The ancestry of African Americans is predominantly from Niger-Kordofanian (~71%), European (~13%), and other African (~8%) populations, although admixture levels varied considerably among individuals. This study helps tease apart the complex evolutionary history of Africans and African Americans, aiding both anthropological and genetic epidemiologic studies.

Right off the bat, the first thing that probably jumps at any reader, is the heavy reliance on microsatellite repeats, as opposed to uniparental binary markers. Restricted fragment length nucleotide sequences or polymorphisms [relying on insertions/deletions] can add precision to identifying discrete monophyletic units, but even here, the results are not always as clear cut as the case generally is with "classic" uniparental binary markers of Y-DNA and mtDNA respectively, relying on unique event SNPs. For instance, certain RFLP haplotypes have been linked to two different haplogroups; case studies on RFLP haplotypes on this website have shed light to such occurrences: see Lucotte et al.'s haplotype IV, RFLPs: Lucotte et al., A case study — Pt. 1, and RFLPs: Lucotte et al., A case study — Pt. 2. In the first link of the three for instance, we were told:

A total of 21 different 49a,f haplotypes were found and are illustrated in Fig. 4 as a sub-classification of the Iraqi Y-chromosome haplogroups. The most represented haplotype of haplogroup E is haplotype 5 (A 2 C 0 D 0 F 1 I 1 ). This is followed by haplotype 11 (A 3 C 0 D 0 F 1 I 1 ) at a much lower frequency. Haplotypes 5 and 11 were observed both in Africa (Lucotte et al., 2001; Passarino et al., 1998; Persichetti et al., 1992; Santachiara-Benerecetti and Semino, 1996; Spurdle and Jenkins, 1992; Torroni et al., 1990) and Eurasia (Passarino et al., 2001; Semino et al., 2000b) but in Africa they belong to haplogroup E, whereas in Eurasia, particularly in *Northeastern Eurasia*, they belong mainly to the haplogroup R-M17. Interestingly, the proportion of haplotypes 5 and 11 in haplogroups E and R-M17 is reversed, with haplotype 5 prevalent in haplogroup E and haplotype 11 in haplogroup R-M17. By considering that the two haplotypes differ by a single band change and their different proportion in the two lineages, it is likely that haplotype 11 is a derivative of haplotype 5 in haplogroup E and just the opposite in haplogroup R-M17. - N. Al Zahery et al. 2003, Y-chromosome and mtDNA polymorphisms in Iraq, a crossroad of the early human dispersal and of post-Neolithic migrations.

In cases where it might be difficult to link a RFLP haplotype to a specific sub-clade or else superclade, classic uniparental (Y-DNA or mtDNA) SNP and/or UEP markers are called on, as we see in the aforementioned link 2 and link 3 on Lucotte et al. Questionable allocation(s) of ancestral origin based on "shared" microsatellite markers and/or insertion/deletion markers is therefore bound to occur, as one comes across in the study at hand; ancestry, which would otherwise actually be more a reflection of an ultimate shared common recent ancestry prior to the ethnogenesis of the implicated contemporary groups into recognizable ethnic or socio-geopolitical entities they are known today, as opposed to some recent unidirectional gene flow from one designated contemporary group to another.

Moving onto excerpts of interest...

As some have correctly picked up on, and as the authors themselves openly admit, there are issues with their Dogon sample that need to be ironed out...

Detection of relative pairs: Relative pairs and duplicated samples in the dataset were inferred from the pattern of shared genotypes and population allele frequencies with RELPAIR 2.0.1 ( 6-8) . Because the inclusion of closely related individuals can impact population genetic inferences [e.g. (S9)], we took the conservative approach of excluding individuals inferred to be third degree or more closely related, including inferred relative pairs between regional ethnic populations (e.g. all Tanzanian populations). An exception was made in the case of the Dogon as it is difficult to reliably infer relative pairs in a small sample and the Dogon are highly distinctive and could not be readily merged with other populations to improve allele frequency estimates. Merging the Dogon with other non-Pygmy West African populations inferred four unrelated individuals in the sample, but this may be overly conservative given the distinctiveness of the Dogon sample from other West Africans. Also, the Dogon are the only representatives from Mali in our study and since the sample size is already small we did not want to further reduce the sample size in the analyses, especially if the relative pair estimates were questionable. Therefore, RELPAIR inferred relative pairs among the Dogon were not excluded. In total 737 individuals were removed. Networks of relatives, which in some cases were quite complex, were plotted with neato from the GraphViz software package (S10), which was used to select the minimum number of individuals to exclude to break up networks of relative pairs.

Indeed, the Dogon sample size, which to reiterate what the authors tell us — consisted of candidates who could also possibly be closely related and the only sample that represented Mali, was only one of 9 individuals, a fairly modest size. Keeping this in mind, we are told:

The Dogon from Mali, who speak a Niger-Kordofanian language, cluster with the Saharan and eastern Africans in the NJ tree (Fig. 1), consistent with the results from STRUCTURE analysis, showing considerable Saharan (blue) and Afroasiatic (purple) ancestry, and consistent with oral history of a northern African origin (although it should be noted that the sample size for this population, 9 individuals, is very small and many markers did not amplify well) (Fig. S13; Table S9).

"Saharan" without regional specificity is next meaningless. Just about any of the groups on the Sahara, Sahel or adjoining "sub-Saharan" territories have "Saharan ancestry". Furthermore, as has been the talk of this site recently, relics of "fertile-Sahara" era or earlier east African ancestry is scattered along the length of the Sahara, and generally finds expression in Sahelian and "Sub-Saharan" populations in relatively low incidences, if not somewhat patchy distribution. Such shared Saharan ancestry is bound to have some sort of manifestation across bi-parental microsatellite markers as well. As we've just seen, the authors note that "many markers did not amplify well", and indeed, if there is any further indication of an air of dubiousness about the results of the amplifications, then perhaps it is notion of some 45% ancestry, or specifically 45% of the sample identified with an AAC (Associated Ancestral Cluster) , being suggestive of European ancestry in that small sample of Dogon, with 9 individuals [see table S8 of the publication]; and even if one were to remotely entertain the possibility as a hypothetical scenario for argument's sake, it would implicate these individual as essentially "outliers", if anything, based off on previous information published on samples taken from sections of Malian society, including the Dogon. Additionally, with regards to such an outlier situation, one might even ask if this is not a function of "third party" interaction, with that "third party" being coastal north African Imazighen and Arabized groups. The authors themselves base AAC assignment on where said markers are allegedly the most maximum in 'frequency'...

African and non-African Associated Ancestral Clusters (AACs; labeled based on the populations showing the highest levels of ancestry for each inferred ancestral cluster)are highly divergent.

One can suppose 'frequency' could be a telltale sign of geographical origin of certain biparental microsatellite clusters, if only tenuously by itself, but as a matter of precision, it effectively plays that role only when taken into account with other molecular genetics information, like the internal molecular variation of a designated clade, and in association, how much of this variation is paraphyletic, along with definite geographical patterns of distribution. For instance, one might surmise that Hg R1b originated in Europe, merely on the account of the haplogroup's unparralleled prevalence in that region, and place the maximum likelihood of finding ancestral R1b clades in Europe before finding them elsewhere. However, as a matter of merely "frequency", Hg R1b's incidence in Europe in no way allows inference that either Hg R1* or its descendant, the upstream clade R1b* originates there; in fact, R1b* clades bearing upstream SNPs that are nearer to the root of that sub-clade, but lacking the well-known downstream SNPs, have so far only been identified in non-European samples. Interestingly, these non-European samples happen to be African ones. At the same, ironically, R1b itself is relatively rare in Africa, even in the coastal north African areas which are closer to Europe. So frequency as a single factor for adjudging origin can be misleading. Neutral factors like random genetic drift, for example, can elevate a marker's frequency in a designated locale, and yet, this locale in of itself need not be the actual location of origin for said marker. For microsatellite repeats, in absence of binary or UEPs, the assignment of origin based on frequency alone becomes even more uncertain, than the case is for binary or SNP markers. Microsatellite repeats could even be shared both between different and within single monophyletic clades in some occasions, due to homoplasy.

Many questions abound the objectivity of result inferred for the small Malian sample. In a related matter, the Beja samples were implicated in some ~ 32% and ~ 34% AACs [the Banuamir and Hadandawa samples respectively] placed under "European" [see: Table S8 of the journal]. Well, to the extent that ancestry suggestive of "possible" European origin has been implicated in a Beja sample before, it is reasonable to assume that this may render a section of the Beja to display shared clusters with Europeans in a segment of nuclear DNA that is outside uniparental binary markers; however, it is worth noting that the uniparental gene pool, the paternally-inherited one in particular, featured fairly low incidence of ancestry suggestive of ultimate European ancestry. Citing Hassan et al. (2008), the Beja male gene pool comprised of only ~ 5% ancestry that could *potentially* be linked to European origin [Hassan et al. (2008) provide little information on specific downstream clades of Hg R1b]; this is fairly consistent with R1b's general distribution in northern Africa, where it is generally low, in contrast to the fairly considerable representation of European maternal ancestry in coastal northwest Africa. So, the 32 or so % of microsatellite AAC linked to a possible European origin is out of character, when uniparental ancestry information is taken into account. On the other hand, substantial AAC sharing between coastal northwest African samples and the European ones is consistent with uniparental DNA examinations, which to reiterate, mainly finds expression in the maternally-inherited gene pool.

Mitochondrial DNA analysis indicates that Fulani have lineages of predominantly West African origin and that they cluster together and close to the Mandenka population from Senegal [S93].

By contrast, Y chromosome analyses of Fulani
sampled in the Sudan indicates shared ancestry with Nilo-Saharan and Afro- Asiatic speaking populations [S89].

The line about the mtDNA is essentially a reversion back to what has been cited in the main post above, as observed by Cerny et al. (2006). The line about the Y chromosome analysis however, is quite misleading; one only needs to refer to Hassan et al. (2008) [see: Y-Chromosome Variation Among Sudanese] to see how: The Sudanese Fula sample here, as noted in the main post above, predominantly featured Hg R1*-M173 markers in the uniparental paternal gene pool. This marker is in fact generally quite rare in "Afro-Asiatic" speaking populations; rather, its highest incidences has thus far been reported in Niger-Congo, Nilo-Saharan and "Afro-Asiatic" (Chadic) speaking groups in northern Cameroon, with smaller occurrences in the rest of central Africa [see Luis et al. 2004]. Nilo-Saharan groups in Sudan itself, it should be noted, did NOT share this ancestry with the Sudanese-based Fulbe at all. This reverts back to what had already put forward in the main post, about the implication being that these Sudanese Fula derived from a section of the northern Cameroonian Fula, wherein the Hg R1*-M173 is featured in a considerable but not the predominant frequency.

The only marker that the Sudanese Fula shared with Nilo-Saharan and Afrasan ("Afro-Asiatic") speaking Sudanese groups was Hg E3b1 (M78). Common sense however, intimates that this aspect of the paternal gene pool was largely picked up from neighboring populations, only after the Fula situated themselves in Sudan. It makes sense, since after all, E3b1 is one of the prominently featured markers found in the Sudanese sample.

To recap, there's something still unmistakable about the Sudanese Fula sample: they retain western African ancestry, as signified by Hg E1-M33, which was absent in all autochthonous Sudanese groups; the only other groups where this marker was implicated in Hassan et al.'s (2008) work, were, well, the well-established west African groups like the Hausa and Wolof. When pressed on to explain away this anomalous feature of the Sudanese Fula's paternal gene pool, dissidents of the west African origin noticeably become mute on the matter.

These results raise the possibility of differential patterns of male and female gene flow into this population.

Whatever may be "differential" about male and female gene flow patterns in Fula sample, no doubt influenced by matters like life style — recalling on nomadic vs sedentary, both the maternal and paternal gene pools communicate the same thing: west African origin!

Our analysis, using genome-wide nuclear markers and STRUCTURE, indicates that the Fulani have distinctive ancestry [fuchsia] at K = 14 in the global analysis [Figs. 3,4] and at K = 9 -14 in the Africa analysis [Fig. S13]. The Fulani cluster with the Chadic and Central Sudanic speaking populations at K <13> They also cluster near the Chadic and Central Sudanic speaking populations in the NJ tree based on population genetic distances [Fig. 1].

Fula samples taken from either central Africa or Sudan are expected to cluster with groups in central Africa-Chad, as the Fula would have arrived in Sudan via a central African and/or Chadic corridor, having experienced a bottleneck [see main blog post]. The central African Fula in turn would have arrived ultimately from Atlantic-coastal regions of western Africa, where their ethnogenesis as the Fula-"proper" likely occurred.

The pattern of the Sudanese Fula's Y-DNA composition is for instance, undoubtedly different from their western African brethren, but continuity still exists via recurring typical western African markers that unite all Fula groups, regardless of where they spread to; e.g. primarily Hgs E1 and E3a, and a lesser extent, Hg E2. Either of these clades are telltale signs of so-and-so Fula groups' western African ancestry.


In the global STRUCTURE analysis, the Fulani show low to moderate levels of European/Middle Eastern ancestry [blue], consistent with mtDNA and Y chromosome [S89] analyses, as well as the presence at low frequency of the -13910 mutation associated with lactose tolerance in Europeans in this population [S94].

Again quite misleading. Yes, understandably, "low" levels of shared ancestry attributable to potential "European" ancestry, might be correlated with "low level" incidences (~1.7 %) of R1b [pertaining the Rosa et al.'s (2007) Guinean sample] or relics of this ancestry attained from coastal northwestern populations [Imazighen] seen on the mtDNA side [Cerny et al. 2006], as cited in the main post. However, in the case of Fula samples, from northern Cameroon through to Sudan, a good degree of presumed shared "European/Middle Eastern ancestry [blue]" may very well actually be linked to common "distant" ancestry, as the paraphyletic R*-M207 and R1*-M173 markers suggest; these latter two are essentially rare to absent in "Middle Eastern" and "European" populations, which are mainly characterized by more downstream markers, especially European populations, who have no upstream markers for R1. Now, because R1b and R1a markers are still 'molecularly' linked to the upstream R*, any group bearing the latter or else any intermediary nodes between the upstream R* and the downstream R1a and R1b will "appear" to share ancestry with Europeans and/or possibly "Middle Easterners", which could misleadingly be misread as ancestry from "European/Middle East". Given the features of northern Cameroonian and Sudanese Fula sample uniparental male gene pool, it is a safe bet that this is the underlying issue at work, especially when the red flag of "moderate" levels is evoked; the Fula uniparental gene pool specifics, whether maternal or paternal, simply do not bear out "moderate" ancestry from "Middle East" or "Europe".

Recalling a previous blog post here
, R1*-M173 in Africa, we have:

Interestingly, upon revisiting Wood et al. (2005), it should be pointed out that paraphyletic clade of R*-M207 was detected amongst some "Afro-Asiatic" African groups, along with the paraphyletic clade R1*-M173 [it is worth noting that Wood et al. implicate the Egyptian sample here as something other than that of Semitic speakers (Arabic)], while some Niger-Congo groups — though in small frequencies [pooled] — tested positive for the paraphyletic R1b*, lacking the established downstream R1b markers. Henceforth, R*-M207, lacking downstream mutations have been identified in African groups via this study; and yes, the basic nodes of all presently known Hg R's downstream clades had been accounted for, which means that R*, as predicted above, is NOT relegated to the Indian sub-continent. All in all, this suggests that African Hg R pool is actually more diverse than many seem to think.

As for the matter concerning lactose tolerance, it has been pointed out here before, how this has nothing to do with European ancestry. Recap, see:
More on R1*-M173 bearers

In summation, the piece essentially rehashes the well-understood facts of primary west African ancestry of the Fula, both in terms of genealogy and language, along with genealogical "admixture" elements picked up over the course of migratory events, but suffers from matters, as laid out in detail in the main blog post, pertaining to idealistic urges for mystification where none is necessary or warranted. To provide an example, consider the following line:

Our analysis, using genomewide nuclear markers and STRUCTURE, indicates that the Fulani have distinctive ancestry (fuchsia) at K = 14 in the global analysis (Figs. 3,4) and at K = 9 -14 in the Africa analysis (Fig. S13). The Fulani cluster with the Chadic and Central Sudanic speaking populations at K <13>Fulani show low to moderate levels of European/Middle Eastern ancestry (blue), consistent with mtDNA (S93) and Y chromosome (S89) analyses, as well as the presence at low frequency of the -13910 mutation associated with lactose tolerance in Europeans in this population (S94). Additionally, we observe moderate to high levels of Niger- Kordofanian ancestry in the Fulani populations (Figs. 3, 4, S13;Tables S8, S9). These results do not enable us to determine the definitive origin of the Fulani, although they indicate shared ancestry with Saharan and Central Sudanic populations and suggest that the Fulani have admixed with local populations, and possibly adopted a Niger-Kordofanian language, during their spread across central and western Africa. The origin of European (possibly via the Iberian peninsula) and/or Middle Eastern ancestry in the Fulani requires further exploration with additional genetic markers.
 

The words "admixed with local populations" is particularly interesting, as it is naturally inconsistent with mtDNA report [and Y-DNA] where western Africa is concerned, recalling Cerny et al. (2006) for example, who point out that their results show a primarily or predominantly west African ancestry of "charasteristic" markers of the Fula, but wherein the gene pool of nomadic Fula samples show very little genetic contribution from neighboring sedentary populations. This, to put it simply, means that the only way for the Fula gene pool to be primarily west African, is if they were autochthonously west African.

Another interesting choice of words, is the mention of "adopt" in Tishkoff et al.'s piece above, in relation to the Fulani language. "adopt" is usually suggestive of one taking on something that wasn't originally his/her's. Supposing one remotely entertained said insinuation of the authors for just a minute, their very idea that a small band of foreigners adopted the "Niger-Kordofanian" language along their migratory path, means that the locals along that path would have had to have already been Fula speakers; that is the only way a wandering group of immigrants/foreigners could have become speakers of a Niger-Congo language — a language family that supposedly did not characterize their own — which by chance, happens to be Fula. In other words, the Fula as an ethnic group was already established on the continent prior to the intrusion of this small band of immigrants; it's just common sense. Henceforth, this band of immigrants cannot be judged as proto-Fula antecedents by any stretch of the imagination; rather, they should be viewed merely as a foreign element that integrated into an already well established ethnic-group, i.e. the Fula, as opposed to assuming the role of a "prototype" for the group into which they allegedly integrated. To somehow use this [unsupported] scenario as something that mystifies Fula origins, is clearly not rational or logical deductive reasoning; at best, it is quite comical. The matter of Fula origins was recently posted here, in detail; recap: The Creation of a Fake Controversy: The Fula Origins

It is interesting to note that while the authors carry themselves as very confused individuals about Fulani ancestry, their own tables (see S8) show very little AAC sharing with Europeans; their Nigerian Fulani sample supposedly shared ~5 % or so AACs placed under "European", while the Cameroonian Fula samples shared about 2.5% and 2.6% respectively. Nothing "moderate" about these.

From language, culture to genealogical heritage, all indicators resolutely point to a west African ethnogenesis of the Fula. This has been supplemented with archaeological evidence, as that from Tassili, on rock art. Here, are renditions of a cattle-rearing group, bearing features reminiscent of contemporary Fula and/or western Sahel groups in general. One scholar often cited on the internet, regardless of how objective or otherwise the sites themselves are, for identifying definite links between the Tassili n'Ajjer rock painting and contemporary Fula, is Amadou Ba. For instance, one site notes:

At the Tin Tazarift site, for instance, historian Amadou Hampate Ba recognized a scene of the lotori ceremony, a celebration of the ox's aquatic origin. In a finger motif, Ba detected an allusion to the myth of the hand of the first Fulani herdsman, Kikala. At Tin Felki, Ba recognized a hexagonal carnelian jewel as related to the Agades cross, a fertility charm still used by Fulani women. With the disappearance of many traditions and other aspects of African culture, works of traditional African art are becoming more and more scarce. - This particular piece is from Jamtan.com

The following are from Andrew Brown Smith [African Herders (2005)], who seemingly attempts to portray himself as a "voice of moderation":






Of note here, is the observation that Smith — as any other person guided by reasoning — could not pass over the significance of linguistics, i.e. recalling the Niger-Congo familial ties of Fula language.



While briefly citing examples of the sort of reception Ba's definite correlations got in certain quarters, mainly amongst "western" observers, even Smith could not resist pointing out that the "coincidences" surrounding the reminiscence of Fula socio-cultural traits in definite features of the rock arts are simply too considerable to not draw a link between the two, i.e. between the figures in the rendition and contemporary Fula. Notwithstanding this openly-admitted acknowledgment, Smith of course, goes onto characterize Ba's demonstrations as "perhaps what they wanted to see to support their thesis", but offers no substantive counter-thesis that puts to question the "specifics" of what Ba "sees", other than a mere opinionated presumption of Ba's "possible" motive behind making the definite links that he made.





Click on the above images to get higher resolution versions.

In the lower images for example, one notices the head gear, which is not unlike that featured on a contemporary Dogon dancer in the image on the right hand side.

Other notes: Fulani cattle are also deemed to be of west African origin, as a sub-phyla of the west African breed of Zebu cattle called the "West African Zebu", and they fall into the following two groups -

The Fulani have been classified further into two groups: the lyre-horned subgroup consisting of Senegalese Fulani (or the Gobra), the Sudanese Fulani, and the White Fulani (or Bunaji); and long-horned subgroup represented by the Red Fulani (or Rahaji). Diali (or Djeli) is a strain of Fulani found on the flood plains of Niger river in Niger and south-west Nigeria (Rege 1999; Rege and Tawah, 1999). - courtesy cdad-is.org

Dictionary description...

Sudanese Fulani cattle
West African, lyre-horned, milking cattle, usually light gray.- Medical-dictionary

Continuing with examination of the study at hand, the authors note:

Individuals from Saharan and Eastern Africa show heterogeneous ancestry, reflecting descent from populations ancestral to non-Africans and/or gene flow from non-Africans into Africa.

Well, "exotic" gene flow as function of said heterogeneity, while reasonably a contributing element to some extent, is really quite trivial in the big scheme of things. African populations would still be quite heterogeneous *regardless* of gene flow from non-Africans
. In relation to this, a subset of Africans who formed the basis for non-African groups, would assume an intermediary position in a case study involving a "comprehensive" collection of African and some non-African samples, because quite simply, that subset of Africans only represents a portion of the overall African diversity. Non-Africans in turn, only have a portion of the diversity of the subset of Africans from whom they emanate.

*Watch for occasional updates or modifications of this post in future.



*Last edited on 10/26/2010.